| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Seavers-Reale, Ivy R (10)
|
MDNBAC |
28.75 |
32.94 |
4.19 |
| 2 |
Harberts, Anna M (9)
|
MDNBAC |
29.80 |
33.12 |
3.32 |
| 3 |
Savage, Tireni C (10)
|
MDNBAC |
30.75 |
34.77 |
4.02 |
| 4 |
Ma, Katelyn X (10)
|
MDNBAC |
30.31 |
34.97 |
4.66 |
| 5 |
Shkolnik, Alexandra N (10)
|
MDNBAC |
30.81 |
35.01 |
4.20 |
| 6 |
Karp, Olivia N (9)
|
MDNBAC |
32.61 |
36.14 |
3.53 |
| 7 |
Schmitt, Leyla G (9)
|
MDNBAC |
32.35 |
36.24 |
3.89 |
| 8 |
Epperson, Eden R (10)
|
MDFSC |
34.78 |
38.39 |
3.61 |
| 9 |
Courtois, Chloe C (9)
|
MDFSC |
32.75 |
38.45 |
5.70 |
| 10 |
Chaudhary, Nevika (10)
|
MDMAS |
34.00 |
39.11 |
5.11 |
| 11 |
Steele, Taylor B (10)
|
MDFSC |
34.76 |
39.12 |
4.36 |
| 12 |
Tomlinson, Scarlet B (10)
|
MDFSC |
31.73 |
39.60 |
7.87 |
| 13 |
Scott, Parker H (9)
|
MDFSC |
37.08 |
40.59 |
3.51 |
| 14 |
Segear, Madison E (10)
|
MDFSC |
35.81 |
41.01 |
5.20 |
| 15 |
Ming, Abigail J (9)
|
MDFSC |
39.08 |
43.22 |
4.14 |
| 16 |
Mitchell, Emma R (10)
|
MDMAS |
37.88 |
44.56 |
6.68 |